14/4/2010
Abstract
A preliminary study was made to evaluate the antagonistic effeciency and mycoparasitic activity of Trichoderma reesei against Botrytis species, viz. B. cinerea, B. allii and B. fabae. The bioassay method to evaluate the efficacy of Trichoderma reesei was the reduction of mycelial growth of Botrytis spp. in vitro. Significant effect was observed against B. cinerea and B. fabae, however, no effect was found against B. allii. Hyphal and sclerotial parasitism of Trichoderma reesei against Botrytis spp. were also studied. Coiling of hyperparasite fungus, penetration into B. cinerea hyphae, and stunt of the latest were observed as typical signs of mycoparasitism. Coiling of B. allii and stunted hyphae of B. fabae were the only signs of hyphal parasitism observed. The sclerotia of B. cinerea were also parasitized but sclerotia of B. allii and B. fabae were not.
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