الابحاث العلمية الدولية بالقسم الخاصة ب أ.د. أشرف محمد أحًمد

A. Papers in Impacted International Journals

1.      Khalifa, H.O., Soliman, A.M., Saito, T., Kayama, S., Yu, L., Hisatsune, J., Sugai, M., Nariya, H., Ahmed, A.M., Shimamoto, T. and Matsumoto, T., (2020). First report of foodborne Klebsiella pneumoniae co-harboring blaVIM-1, blaNDM-1, and mcr-9. Antimicrob Agents Chemother, 64, pp.e00882-20. (Impact factor 4.415).

2.      El-Tawab, A.A.A., Ahmed, A.M., Nabih, A.M. and Saad, W.H. (2020). First characterization of class 1 integron in Corynebacterium bovis isolated from subclinical bovine mastitis. Adv. Anim. Vet. Sci, 8(5), pp.452-457.

3.      Khalifa, O.H., Soliman A.M., Ahmed A.M., Shimamoto T., Nariya, H., and Shimamoto T. (2019) High Prevalence of Antimicrobial Resistance in Gram-Negative Bacteria Isolated from Clinical Settings in Egypt: Recalling for Judicious Use of Conventional Antimicrobials in Developing Nations. Microbial Drug Resistance 25 (3), 371-385. (Impact factor 2.344).

4.      Soliman, A. M., Saad, A. M., Ahmed, A. M., Shimamoto, T., Nariya, H., and Shimamoto, T. (2018) Occurrence of Salmonella genomic island 1 (SGI1) in two African Proteus mirabilis strains isolated from diseased chicken flocks. Infection, Genetics and Evolution, 62, 8-10.‏ (Impact factor 2.885).

5.      Khalifa, O.H., Soliman A.M., Ahmed A.M., Shimamoto T. and Shimamoto T. (2017) High Carbapenem Resistance in Clinical Gram-Negative Pathogens Isolated in Egypt. Microbial Drug Resistance, 23(7):838-844. (Impact factor 2.344).

6.      Soliman, A. M., Ahmed, A. M., Shimamoto, T., El-Domany, R. A., Nariya, H., & Shimamoto, T. (2017). First report in Africa of two clinical isolates of Proteus mirabilis carrying Salmonella genomic island (SGI1) variants, SGI1-PmABB and SGI1-W. Infection, Genetics and Evolution, 51, 132-137.‏ (Impact factor 2.885).

7.      Khalifa, O.H., Ahmed A.M., Oreiby, A.F., Eid, A.M., Shimamoto T. and Shimamoto T. (2016) Characterisation of the plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli isolated from animals in Egypt. International journal of antimicrobial agents 47 (5), 413. (Impact factor 4.307).

8.      Elnahriry S.S., Khalifa, O.H., Soliman A.M., Ahmed A.M., Moustafa A.H., Shimamoto T. and Shimamoto T. (2016) Emergence of plasmid-mediated colistin resistance gene, mcr-1, in a clinical Escherichia coli isolate from Egypt. Antimicrobial Agents and Chemotherapy 60:3249-3250. (Impact factor 4.415).

9.      Khalifa, O.H., Soliman A.M., Ahmed A.M., Shimamoto T. and Shimamoto T. (2016) NDM-4- and NDM-5-Producing Klebsiella pneumoniae coinfection in a 6-month-old infant. Antimicrobial Agents and Chemotherapy 60:4416-4417. (Impact factor 4.415).

10.  Soliman A.M., Khalifa, O.H., Ahmed A.M., Shimamoto T. and Shimamoto T. (2016) Emergence of an NDM-5-producing clinical Escherichia coli isolate in Egypt. International Journal of Infectious Diseases 48, 46-48. (Impact factor 2.229).

11.  Ahmed A.M., Maruyama A., Khalifa, O.H., Shimamoto T. and Shimamoto T. (2015) Seafood as a reservoir of Gram-negative bacteria carrying integrons and antimicrobial resistance genes in Japan. Biomedical and Environmental Sciences, 28 (12), 924-926.  (Impact factor 2.204).

12.  Ahmed A.M., and Shimamoto T. (2015) Molecular characterization of multidrug-resistant Shigella spp. of food origin. International Journal of Food Microbiology, 194: 78–82.

13.  Ahmed A.M., and Shimamoto T. (2015) Molecular analysis of multidrug resistance in shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products. International Journal of Food Microbiology, 193: 68–73. (Impact factor 3.339).

14.  Ahmed A.M., Shimamoto T. and Shimamoto T. (2014) Characterization of integrons and resistance genes in multidrug-resistant Salmonella enterica isolated from meat and dairy products in Egypt. International Journal of Food Microbiology, 189: 39– 44. (Impact factor 3.339).

15.  Ahmed  A.M., and Shimamoto T. (2014) Isolation and molecular characterization of Salmonella enterica, Escherichia coli O157:H7 and Shigella spp. from meat and dairy products in Egypt. International Journal of Food Microbiology, 168–169: 57–62. (Impact factor 3.339).

16.  Ahmed A.M., Shimamoto T. and Shimamoto T. (2013) Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. International Journal of Medical Microbiology, 303: 475– 483. (Impact factor 3.339). (Impact factor 3.391).

17.  Shimamoto T., Ahmed A.M., and Shimamoto T. (2013) A novel retron of Vibrio parahaemolyticus is closely related to retron-Vc95 of Vibrio cholerae. Journal of Microbiology, 51(3):323-328.

18.  Ahmed A.M. and Shimamoto T. (2012) Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt. Microbiology and Immunology, 56 (4):254-261.

19.  Ahmed A.M. and Shimamoto T. (2011) Molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from bovine mastitis in Egypt. Microbiology and Immunology, 55(5):318-327.

20.  Ishida Y, Ahmed A.M., Mahfouz NB, Kimura T, El-Khodery SA, Moawad AA and Shimamoto T. (2010) Molecular Analysis of Antimicrobial Resistance in Gram-Negative Bacteria Isolated from Fish Farms in Egypt. Journal of Veterinary Medical Science, 72 (6):727-734.

21.  Sato M, Ahmed A.M., Noda A, Watanabe H, Fukumoto Y and Shimamoto T. (2010) Isolation and molecular characterization of multidrug-resistant Gram-negative bacteria from imported flamingos in Japan. Acta Vet. Scand., 51:46.

22.  Ahmed A.M., Shimabukuro H and Shimamoto T. (2009) Isolation and molecular characterization of multidrug-resistant strains of Escherichia coli and Salmonella from retail chicken meat in Japan. Journal of Food Science, 74: M405-M410.

23.  Ahmed A. M., Younis EE, Ishida Y and Shimamoto T. (2009) Genetic basis of multidrug resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from diarrheic calves in Egypt. Acta Tropica, 111:144-149.

24.  Ahmed A.M., Younis EE, Osman SA, Ishida Y, El-Khodery SA and Shimamoto T. (2009) Genetic analysis of antimicrobial resistance in Escherichia coli isolated from diarrheic neonatal calves. Veterinary Microbiology, 136:397-402.

25.  Ahmed A.M., Ishida Y. and Shimamoto T. (2009) Molecular characterization of antimicrobial resistance in Salmonella isolated from animals in Japan. Journal of Applied Microbiology, 106 (2): 402-409.

26.  Hussein AI, Ahmed A.M., Sato M and Shimamoto T. (2009) Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals. Microbiology and Immunology, (11):595-602.

27.  Younis EE, Ahmed A.M., El-Khodery SA, Osman SA, El-Naker YF. (2009) Molecular screening and risk factors of enterotoxigenic Escherichia coli and Salmonella spp. in diarrheic neonatal calves in Egypt. Research in Veterinary Science, 87: 373-379.

28.  Hammad AM, Ahmed A.M., Ishida Y and Shimamoto T (2008) First characterization and emergence of SHV-60 in raw milk of a healthy cow in Japan. Journal of Veterinary Medical Science, 70 (11):1269-1272.

29.  Ahmed A.M. and Shimamoto T. (2008) Emergence of a cefepime- and cefpirome-resistant Citrobacter freundii clinical isolate harbouring a novel chromosomally encoded AmpC beta-lactamase, CMY-37. International Journal of Antimicrobial Agents 32(3):256-261.

30.  Ahmed A.M., Motoi Y, Sato M, Maruyama A, Watanabe H, Fukumoto Y and Shimamoto T. (2007). Zoo animals as reservoirs of gram-negative bacteria harboring integrons and antimicrobial resistance genes. Applied and Environmental Microbiology, 73:6686-6690.

31.  Ahmed A.M., Hussein AI and Shimamoto T. (2007) Proteus mirabilis clinical isolate harbouring a new variant of Salmonella genomic island 1 containing the multiple antibiotic resistance region. Journal of Antimicrobial Chemotherapy, 59:184-190.

32.  Ahmed A.M., Kawamoto H, Inouye K, Hashiwata Y, Sakaki M, Seno M and Shimamoto T. (2006) Genetic characterization of multidrug resistance in Shigella spp. from Japan. Journal of Medical Microbiology, 55:1685-1691.

33.  Ahmed A.M., Kawaguchi F and Shimamoto T. (2006) Class 2 integrons in Vibrio cholerae. Journal of Medical Microbiology, 55:643-644.

34.  Ahmed A.M., Furuta K., Kawamoto H., Inoue K., Hashiwata Y. Sakaki M., Seno M. and Shimamoto T. (2005) Genomic analysis of a multidrug-resistant strain of enterohaemorrhagic Escherichia coli O157:H7 causing a family outbreak in  Japan. Journal of Medical Microbiology, 54:867-872.

35.  Ahmed A.M., Furuta K., Shimomura K., Kawamoto H. and Shimamoto T. (2005) Characterization of a multidrug-resistant isolate of Salmonella Paratyphi B from Japan. Journal of Antimicrobial Chemotherapy, 56:250-250a.

36.  Ahmed A.M., Nakano H. and Shimamoto T. (2005) Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron. Journal of Antimicrobial Chemotherapy, 55:371-374.

37.  Ahmed A.M., Miyoshi S., Shinoda S. and Shimamoto T. (2005) Molecular characterization of a multidrug-resistant strain of enteroinvasive Escherichia coli O164 isolated in Japan. Journal of Medical Microbiology, 54:273-278.

38.  Ahmed A.M., Shinoda S. and Shimamoto T. (2005) A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis. FEMS Microbiology Letters, 242:241-247.

39.  Ahmed A.M., Nakano H. and Shimamoto T. (2004) The first characterization of extended-spectrum β-lactamase-producing Salmonella in Japan. Journal of Antimicrobial Chemotherapy, 54:283-284.

40.  Ahmed A.M., Nakagawa T., Arakawa E., Ramamurthy T., Shinoda S. and Shimamoto T. (2004) New aminoglycoside acetyltransferase gene, aac(3)-Id, in a class 1 integron from a multiresistant strain of Vibrio fluvialis isolated from an infant aged 6 months. Journal of Antimicrobial Chemotherapy, 53:947-951.

41.  Ahmed A.M. and Shimamoto T. (2004) A plasmid-encoded class 1 integron carrying sat, a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan. FEMS Microbiology Letters, 235:243-248.

42.  Ahmed A.M. and Shimamoto T. (2003) msDNA-St85, a multicopy single-stranded DNA isolated from Salmonella enterica serovar Typhimurium LT2 with the genomic analysis of its retron.  FEMS Microbiology Letters, 224:291-297.

B- PAPERS and posters IN International Conferences

43.  Shimamoto T., Ahmed A.M., Maruyama A. and Shimamoto T. (2015) Characterization of integrons and antimicrobial resistance genes in Gram-negative bacteria isolated from seafood in Japan: identification of a novel β-lactamase-encoding gene, bla_CMY-39. World Congress and Exhibition on Antibiotics. September 14-16, 2015 (Las Vegas, USA).

44.  Ahmed A.M. and Shimamoto T. (2014) Food Security and Food Safety in Asia”. 7th International Symposium on Food and Environment (Hiroshima, Japan).

45.  Ahmed A.M. and Shimamoto T. (2008) Isolation and molecular characterization of multidrug resistant strains of Escherichia coli and Salmonella from retail chicken meat in Japan. 1^st ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens (Copenhagen, Denmark).

46.  Ahmed A.M., Motoi Y, Sato M, Maruyama A, Watanabe H, Fukumoto Y and Shimamoto T. (2007) Zoo animals as a potential reservoir of gram-negative bacteria harboring integrons and antimicrobial resistance genes. 13^th International Symposium for the World Association of Veterinary Laboratory Diagnosticians (Melbourne, Australia).

47.  Ahmed A.M., Kawamoto H, Inouye K, Hashiwata Y, Sakaki M, Seno M and Shimamoto T. (2006) Molecular characterization of multidrug resistance in Shigella spp. from Japan.10^th Western Pacific Congress on Chemotherapy and Infectious Diseases (Fukuoka, Japan).

48.  Ahmed A.M., Kawaguchi F and Shimamoto T. (2006) Class 2 integrons in Vibrio cholerae. The 41^th Joint Conference of US-Japan cooperative medical science program cholera and related diarrheal diseases panel (Gifu, Japan).

49.  Ahmed A.M., Furuta K, Shimomura K, Kasama Y. and Shimamoto T. (2005) Characterization of antimicrobial resistance mechanisms of Shigella spp. isolated from humans in Hiroshima, Japan. The 40^th Joint Conference of US-Japan cooperative medical science program cholera and related diarrheal diseases panel (Boston, USA).

50.  Ahmed A.M., Furuta K., Kawamoto H., Inoue K., Hashiwata Y. Sakaki M., Seno M. and Shimamoto T. (2005) Molecular characterization of a multidrug- resistant strain of enterohaemorrhagic Escherichia coli O157:H7 causing a family outbreak in Japan. 5^th international Symposium on Antimicrobial Agents and Resistance (Seoul, Korea).

51.  Ahmed A.M., Nakano H and Shimamoto T. (2005) Prevalence and characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron. 5^th international Symposium on AntimicrobialAgents and Resistance (Seoul, Korea).

52.  Ahmed A.M., Shinoda S. and Shimamoto T. (2004) Characterization of a variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis. The 39^th Joint Conference of US-Japan cooperative medical science program cholera and related diarrheal diseases panel (Kyoto, Japan).

53.   Ahmed A.M., Miyoshi S., Shinoda S. and Shimamoto T. (2004) Molecular characterization of a multidrug-resistant strain of enteroinvasive Escherichia coli O164 isolated in Japan. The 7^th Korea-Japan International Symposium on Microbiology (Seoul, Korea).

54.  Ahmed A.M., Nakagawa, T., Arakawa, E.,?Ramamurthy,T., Shinoda, S. and  Shimamoto T. (2003) A new aminoglycoside acetyltransferase gene, aac(3)-Id and aadA7 in class 1 integron from a multiresistant strain of Vibrio fluvialis isolated from a six-month infant with a cholera-like diarrhea in India. The 26^th Annual Meeting of the Molecular Biology Society of Japan (Kobe, Japan).

55.  Ahmed A.M., Nakagawa, T., Arakawa, E., Ramamurthy, T., Shinoda, S. and  Shimamoto T. (2003) Class I integrons and SXT elements in Vibrio cholerae non-O1/non-O139 and Vibrio fluvialis isolated from 2001 to 2002 in India: The identification of a novel aminoglycoside acetyltransferase gene, aac(3)-Id .The 38^th Joint Conference of US-Japan cooperative medical science program cholera and related diarrheal diseases panel (Washington DC, USA).

56.  Ahmed A.M. and Shimamoto T.(2002)  A novel multicopy single-stranded DNA (msDNA) isolated from Salmonella enterica serovar Typhimurium. The 6^th Japan-Korea International Symposium on Microbiology (Suita, Japan).

57.   Shimamoto T., Kageyama N., Shimamoto T., Fujimura M, Ahmed A.M. and Kawakami H. (2002)  Distribution of retron among serogroups of Vibrio cholerae and its integration into the chromosome. The 2002 Meeting on Molecular Genetics of Bacteria & Phages (Cold Spring Harbor, NY, USA).