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Occurrence and incidence of phytoplasmas of the 16SrII-D subgroup on solanaceous and cucurbit crops in Egypt
Research Areafaculty-of-agriculture
Year2012
AuthorsAyman F. Omar
JournalEur J Plant Pathol
Volume
Month
ISSN
AbstractSymptoms reminiscent of phytoplasma infection were observed in four provinces (governo- rates) of Egypt in fields of eggplants, tomato plants and squash. Diseased plants exhibited stunting, leaf yellows and flower development abnormalities. PCR amplification of 16SrDNA with phytoplasma-specific primer pairs confirmed the phytoplasma presence. Sequencing and phylogenetic analysis indicated that all phytoplasmas had the same partial 16SrDNA sequence, assigning them to the 16SrII-D phyto- plasma subgroup. Disease incidence was about 1% among the 20 squash fields surveyed and equally varied from 4% to 15% in the 20 eggplant fields and in the 40 tomato fields inspected. The widespread distribution of this phytoplasma in annual solana- ceous and cucurbit crops suggests a wider plant hostrange including wild plants that could act as reservoir and insist on the need for a insect vector survey. A finer genetic differentiation of Egyptian 16SrII-D phytoplasma strains from different geographical ori- gins and different host plants should help to better trace such epidemics
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